Fig 1: Predicting Cell Signaling within the placental labyrinth.(A) Heatmap showing all Wnt signaling interactions (top) and Vegf signaling (bottom) between the endothelium and labyrinth trophoblast populations. Left: schematics summarizing signaling interactions. (B) Immunofluorescence staining for VEGFA and IGF1R demonstrating colocalization in SynTII in E12.5 mouse placenta sections (63x). Fetal Vessels are outlined in yellow dashed line. (C) Heatmap showing all Egfr signaling interactions between labyrinth populations. Schematic showing secretion of Egf ligands Hbegf, Tgfa, and Nrg1 from SynTI to Egfr expressed on SynTII, LaTP, and S-TGC. (D) Immunofluorescence staining for HBEGF and EGFR demonstrating colocalization of the ligand and receptor SynTII, but only HBEGF expression in SynTI (E12.5 mouse placenta sections - 63x) (E) Heatmap showing signaling interactions of Bmp8a (top) and Bmp7 (bottom), secreted by the LaTP and SynTII or LaTP only, respectively. (F) Heatmap showing predicted signaling interactions between Bmp6 secreted by the endothelium to receptors on LaTP, SynTI, SynTII and S-TGC. (G) Transcript expression for the Smad TFs which are differentially regulated (Smad1/3/5/6).